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2026 fragmentase  (New England Biolabs)


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    Structured Review

    New England Biolabs 2026 fragmentase
    2026 Fragmentase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+dsdna+fragmentase/NEBNext+dsDNA+Fragmentase/pm42872712-116-4-6
    Average 98 stars, based on 753 article reviews
    2026 fragmentase - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Sequencing:

    Article Title: Eupentacta fraudatrix: the first chromosome-level holothurian genome with extraordinarily high heterozygosity.
    Article Snippet: .. Chromosome conformation-captured DNA (3 AR TI CL E IN P RE SS μg) was prepared for sequencing on DNBSEQ-G50 in the PE160 mode with a MGIEasy Universal DNA Library Prep Kit and NEBNext dsDNA Fragmentase. ..

    Article Title: Whole-genome sequence dataset of Pasteurella multocida strain EH32 isolated from buffaloes in Vietnam
    Article Snippet: .. Data collection , Genomic DNA of P. multocida strain EH32 was extracted from pure cultures using the QIAamp DNA Mini Kit. Sequencing libraries were prepared with the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit. Libraries were sequenced using a paired-end (2 × 150 bp) chemistry on the PacBio Onso platform. Genome annotation was performed with Prokka v1.14.6, and functional categories were assigned using the COG and KEGG databases; carbohydrate-active enzymes were identified with dbCAN3.. ..

    Article Title: Whole-genome sequence dataset of Pasteurella multocida strain EH32 isolated from buffaloes in Vietnam
    Article Snippet: The P. multocida strain EH32 was cultured on blood agar and incubated at 37 °C for 24 h. Genomic DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, USA) according to the manufacturer’s instructions. .. Whole-genome sequencing libraries were prepared using the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, USA). ..

    Binding Assay:

    Article Title: NAP-seq for full-length noncapped RNA sequencing.
    Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution.

    Reverse Transcription:

    Article Title: NAP-seq for full-length noncapped RNA sequencing.
    Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution.

    Ligation:

    Article Title: NAP-seq for full-length noncapped RNA sequencing.
    Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution.

    Functional Assay:

    Article Title: Whole-genome sequence dataset of Pasteurella multocida strain EH32 isolated from buffaloes in Vietnam
    Article Snippet: .. Data collection , Genomic DNA of P. multocida strain EH32 was extracted from pure cultures using the QIAamp DNA Mini Kit. Sequencing libraries were prepared with the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit. Libraries were sequenced using a paired-end (2 × 150 bp) chemistry on the PacBio Onso platform. Genome annotation was performed with Prokka v1.14.6, and functional categories were assigned using the COG and KEGG databases; carbohydrate-active enzymes were identified with dbCAN3.. ..

    Enzymatic Fragmentation:

    Article Title: Genome-wide identification of differentially methylated regions in prefrontal cortex of suicide completers in an Indian ancestral admixture population.
    Article Snippet: Objectives: Suicide is a complex phenotype influenced by genetic, environmental, and psychosocial determinants.. Epigenetic mechanisms, particularly DNA methylation, play a critical role in mediating the long-term effects of environmental stressors on gene expression within the brain, potentially contributing to suicide vulnerability.. India represents the largest proportion of global suicide deaths, yet the majority of suicide epigenetics research has focused on individuals of Western admixture.

    Sonication:

    Article Title: Genome-wide identification of differentially methylated regions in prefrontal cortex of suicide completers in an Indian ancestral admixture population.
    Article Snippet: Objectives: Suicide is a complex phenotype influenced by genetic, environmental, and psychosocial determinants.. Epigenetic mechanisms, particularly DNA methylation, play a critical role in mediating the long-term effects of environmental stressors on gene expression within the brain, potentially contributing to suicide vulnerability.. India represents the largest proportion of global suicide deaths, yet the majority of suicide epigenetics research has focused on individuals of Western admixture.

    Qubit:

    Article Title: Patient-Derived Fibroblasts as a Clinically Relevant Model of Kearns-Sayre Syndrome.
    Article Snippet: A mtDNA fragment of 16,267 bp was amplified as previously described13 by long-range PCR using the Takara LA PCR kit (Takara) and the forward (50-ACCGCCCGTCACCC TCCTCAAGTATACTTCAAAGG-30) and reverse (50-A CCGCCAGGTCCTTTGAGTTTTAAGCTGTGGCTC G-30) primers. .. Amplicons were quantified using Qubit 2.0 (Invitrogen), and 1,000 ng were fragmented using NEBNext dsDNA Fragmentase (New England BioLabs) to fragments of approximately 250 bp and cleaned up with Ampure XP magnetic beads (Beckman Coulter). .. Samples were indexed and multiplexed using NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1; New England BioLabs) following the manufacturer’s instructions.

    Magnetic Beads:

    Article Title: Patient-Derived Fibroblasts as a Clinically Relevant Model of Kearns-Sayre Syndrome.
    Article Snippet: A mtDNA fragment of 16,267 bp was amplified as previously described13 by long-range PCR using the Takara LA PCR kit (Takara) and the forward (50-ACCGCCCGTCACCC TCCTCAAGTATACTTCAAAGG-30) and reverse (50-A CCGCCAGGTCCTTTGAGTTTTAAGCTGTGGCTC G-30) primers. .. Amplicons were quantified using Qubit 2.0 (Invitrogen), and 1,000 ng were fragmented using NEBNext dsDNA Fragmentase (New England BioLabs) to fragments of approximately 250 bp and cleaned up with Ampure XP magnetic beads (Beckman Coulter). .. Samples were indexed and multiplexed using NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1; New England BioLabs) following the manufacturer’s instructions.

    Purification:

    Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity
    Article Snippet: .. Adapter-ligated DNA was fragmented using the NEBNext dsDNA Fragmentase (NEB, #M0348S) at 37 °C for 1 h. Reactions were stopped with 0.5 M EDTA, diluted with water, and immediately subjected to SPRISelect purification. ..

    Spectrophotometry:

    Article Title: Draft genome data of Ochrobactrum anthropi strain Nas42 from estuarine sediments of the Red River Delta, Vietnam
    Article Snippet: .. Data collection , Genomic DNA of Ochrobactrum anthropi strain Nas42 was extracted from a metal-selective culture of sediments from the Red River Delta (Vietnam), quality-checked by spectrophotometry and agarose gel electrophoresis, and prepared for whole-genome sequencing using the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, USA). Sequencing was performed on the Illumina MiSeq platform. The raw reads were trimmed using Trimmomatic v0.39, assembled with SPAdes v3.2.0 and Unicycler v0.5.1, and subjected to genome quality assessment with QUAST v5.3.0, BUSCO v5.8.0, and CheckM2 v1.0.2. Functional annotation was carried out using Prokka v1.14.6, COG, KEGG, GO, and RAST, followed by whole-genome-based taxonomic analysis using TYGS, ANIb, and IS711 screening.. .. Data source location , Institute of Biology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.

    Agarose Gel Electrophoresis:

    Article Title: Draft genome data of Ochrobactrum anthropi strain Nas42 from estuarine sediments of the Red River Delta, Vietnam
    Article Snippet: .. Data collection , Genomic DNA of Ochrobactrum anthropi strain Nas42 was extracted from a metal-selective culture of sediments from the Red River Delta (Vietnam), quality-checked by spectrophotometry and agarose gel electrophoresis, and prepared for whole-genome sequencing using the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, USA). Sequencing was performed on the Illumina MiSeq platform. The raw reads were trimmed using Trimmomatic v0.39, assembled with SPAdes v3.2.0 and Unicycler v0.5.1, and subjected to genome quality assessment with QUAST v5.3.0, BUSCO v5.8.0, and CheckM2 v1.0.2. Functional annotation was carried out using Prokka v1.14.6, COG, KEGG, GO, and RAST, followed by whole-genome-based taxonomic analysis using TYGS, ANIb, and IS711 screening.. .. Data source location , Institute of Biology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.

    RAST Test:

    Article Title: Draft genome data of Ochrobactrum anthropi strain Nas42 from estuarine sediments of the Red River Delta, Vietnam
    Article Snippet: .. Data collection , Genomic DNA of Ochrobactrum anthropi strain Nas42 was extracted from a metal-selective culture of sediments from the Red River Delta (Vietnam), quality-checked by spectrophotometry and agarose gel electrophoresis, and prepared for whole-genome sequencing using the NEBNext dsDNA Fragmentase and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, USA). Sequencing was performed on the Illumina MiSeq platform. The raw reads were trimmed using Trimmomatic v0.39, assembled with SPAdes v3.2.0 and Unicycler v0.5.1, and subjected to genome quality assessment with QUAST v5.3.0, BUSCO v5.8.0, and CheckM2 v1.0.2. Functional annotation was carried out using Prokka v1.14.6, COG, KEGG, GO, and RAST, followed by whole-genome-based taxonomic analysis using TYGS, ANIb, and IS711 screening.. .. Data source location , Institute of Biology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.



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